PROBING CONFORMATIONAL STATES OF A TARGET PROTEIN IN ESCHERICHIA COLI CELLS BY IN VIVO CYSTEINE CROSS-LINKING COUPLED WITH PROTEOLYTIC GEL ANALYSIS

Probing Conformational States of a Target Protein in Escherichia coli Cells by in vivo Cysteine Cross-linking Coupled with Proteolytic Gel Analysis

Transporters are dynamic membrane proteins that are essential to the physiology of cells.To function, transporters must cycle between various conformational states, so to understand their mechanistic details, it is critical to characterize how their structure changes during the transport cycle.One approach to studying the dynamics of transporters t

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Research on Gas Injection Limits and Development Methods of CH4/CO2 Synergistic Displacement in Offshore Fractured Condensate Gas Reservoirs

Gas injection for enhanced oil and gas reservoir recovery is a crucial method in offshore Carbon Capture, Utilization, and Storage (CCUS).The B6 buried hill condensate gas reservoir, characterized by high CO2 content, a deficit in natural energy, developed fractures and low-pressure differentials between formation and saturation pressures, requires

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An East Meets West Approach to the Understanding of Emotion Dysregulation in Depression: From Perspective to Scientific Evidence

Depression, an emotion regulation disorder, is a prevalent mental illness in the world.Meanwhile, traditional Chinese medicine (TCM) has been increasingly regarded as a promising and effective alternative therapy approach for patients with depression.Despite many years of research on depression, the current understanding of the pathological mechani

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